2010 NUCLEAR DNA (nuDNA) RESULT DISCUSSED
By Lloyd Pye
Summary
A modified "shotgun" DNA recovery technique has been successfully used to recover coherent segments of the Starchild Skull's nuclear DNA. Of the (approx.) 3 billion base pairs in the skull's genome, several thousand have been recovered. These nuclear DNA fragments have been analyzed by the National Institutes of Health BLAST program, and a substantial percentage of that DNA has "no significant similarity" to any DNA in the extensive database. These results are preliminary and require repetition and verification before they can be considered fully valid, but even at this early stage they are extremely compelling.
Discussion
Early in 2010 the head of a large genetics lab in the U.S. contacted the Starchild Project and suggested that he could attempt to use a recovery technique called modified "shotgun" sequencing to isolate the Starchild's nuclear DNA. This is the same nuclear DNA that could not be recovered during six attempts in an extensive DNA test conducted by Trace Genetics in 2003.
Trace Genetics used what was available then: long human-only primers made from many thousands of base pairs strung together. The new refined shotgun technique could recover much shorter strings of as little as 200 to 500 base pairs long. Where primers are like a single bullet, the new technique is like a spray of shotgun pellets, giving a much better chance to hit a result. The geneticist was certain that if the skull's nuclear DNA was still viable, then, human or not, he could recover it.
A sample of the Starchild Skull's bone was provided, and in a few weeks the geneticist reported some incredible results. Not only had he recovered substantial amounts of nuclear DNA, he had also made a historic discovery when he attempted to catalogue his results. The gel sheet below shows an unmistakable recovery of its nuclear DNA, showing more than a half-dozen strings between 1000 and 2000 base pairs long.
Whenever a geneticist wants to have an unknown sequence of DNA analyzed, they send it for analysis to the enormous genetic database located at the National Institutes of Health (NIH) in Maryland. That public-access database is a centralized repository of all the genomic data accumulated by U.S. government funded research, and it now covers every phylum on Earth, from various kinds of viruses and bacteria, to various kinds of crustaceans and fish, to all kinds of animals and plants, including primates and humans.
Our geneticist sent several samples of the Starchild's nuclear DNA to be compared with trillions of recorded sequences at the NIH. Below we see a report summary returned by the NIH Basic Logical Alignment Search Tool (BLAST) on one 265 base pair long segment of the Starchild Skull's nuclear DNA . In this report we see that it matches perfectly with a gene on human chromosome 1. This verifies that at least some of the nuclear DNA from the Starchild is from a human being.
In the next screen shot (below) a string of 342 base pairs recovered from the Starchild Skull was analyzed. This time the result reads: "No significant similarity found. To have recovered a string of base pairs 342 nucleotides long with NO reference in the NIH database is astounding because the database includes such a wide array of species from bacteria all the way up through the animal kingdom to humans. If there was even a vague similarity in the DNA of any of those species, BLAST would have reported it, but instead "no significant similiary" could be found.
Please understand that this result was repeated and several times with several of the DNA sctions recovered. Strings of Starchild DNA over 3000 base pairs long have failed to match with anything in the NIH database. Despite that, skeptics will be obligated by their positions to try to say it is some kind of genetic gibberish or a mistake made during the analysis process. Why? Because, in the words of philosopher Arthur Schopenhauer: "Every truth passes through three stages. First it is ridiculed. Second it is violently opposed. Third it is accepted as being self-evident."
Luckily, any protest can easily be overcome with continued repetition and reproduction of results, isolating more and more unique fragments to add to the library of data already being created from Starchild DNA.
